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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2011 Aug 24;52(2):410–418. doi: 10.1016/j.yjmcc.2011.08.013

Fig 3.

Fig 3

ZMP (the AICAR metabolite) activates rat ventricular KATP channels without an effect on the unitary conductance. A, Inside-out recordings demonstrating that KATP channels are dose-dependently blocked by cytosolic ATP. There was no effect of 1 mM AICAR when applied in the presence of 100 µM ATP. Similar results were observed in 4 other patches. B, Upper panel: KATP channel activity was partially blocked by 30 µM ATP; subsequent addition of ZMP (100 µM) led to a progressive and reversible increase in KATP channel activity. Lower panel: pre-application of Compound C (10 µM) prevented the ZMP-induced activation of KATP channel activity. C, All-points histograms of KATP channel activity before (control) and after application of ZMP (100 µM) demonstrates that there was no effect on the unitary current amplitude (~5.9pA in this patch). The patch potential was −80mV. The result depicted is representative of 10 similar recordings.