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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2011 Aug 24;52(2):410–418. doi: 10.1016/j.yjmcc.2011.08.013

Fig 6.

Fig 6

Co-immunoprecipitation of AMPK α-subunits and KATP channel subunits. Co-immunoprecipitation of cell lysates from cells transfected with Kir6.2-HA and SUR2A cDNAs and rat ventricular tissue. Detection of AMPKα in the immunoprecipitate obtained with anti-SUR2A antibodies. B, Kir6.2-HA protein was detected in the immunoprecipitate obtained with the anti-Kir6.2 (W62), anti-AMPKα (U) and anti-SUR2A antibodies, but not with anti-GFP antibodies that were used as a negative control. C, Detection of Kir6.2-HA protein in immunoprecipitates obtained with a different anti-Kir6.2 (G16) antibody, as well as the immunoprecipitate obtained with two separate anti-AMPKα antibodies. D, Detection of AMPKα subunits in immunoprecipitates obtained with an anti-Kir6.2 (W62b) antibody. An unrelated antibody (rabbit IgG) was used as a negative control. The results shown are representative of 2–3 separate experiments, which showed similar results.