Fig. 4.

Effect of denervation on wound area. Both denervated and non-denervated ears were wounded with a 2-mm clinical biopsy punch. Denervation of ear wounds caused a significant increase in punch hole area (*P ≤0.05) compared with non-denervated ear wounds from day 7 post-wounding in MRL/MpJ (a) and day 10 in C57BL/6 (b). Ear hole diameter was measured at intervals of 5, 7, 10, 14, 21, 35 and 50 days post-wounding. Image pro plus software was used to calculate wound area. Data shown represent means ± SEM, MRL/MpJ; n = 6, C57BL/6; n = 6 for each time point.