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. 2011 Dec 15;25(24):2631–2643. doi: 10.1101/gad.181289.111

Figure 2.

Figure 2.

The double-mutant form of Arm restores irregular adherens junctions of arm-null clones, but fails to rescue Wg-mediated transcription in vivo. (A,B) Confocal sections of wing discs are shown that contain arm-null (arm−) clones marked by the loss of ubi-GFP expression (borders of clones are highlighted). The particular form of ubiquitously expressed transgenic Arm is indicated in the top corner. Panels to the left show the shape of the clones and the expression of GFP, the middle panels show immunostainings of proteins indicated in the bottom left corner, and the panels to the right show the merged images. (A) Loss of Arm in arm− clones results in defective cell shapes and improper adherens junctions as revealed by the adherens junction component E-cadherin. Ubiquitous expression of wild-type Arm fully restores the normal cell adhesion pattern within arm-null clones (arm−, tubArm-wt). Expression of Arm lacking the ability to bind both Lgs and C-terminal coactivators sustains normal adhesivity, indistinguishable from the wild-type situation. (B) Arm− clones fail to express the Wg target gene senseless (sens). Ubiquitous expression of wild-type Arm (tubArm-wt) fully restores the Sens expression pattern. Arm mutants lacking the ability to bind Lgs (tubArm-D172A) or missing the interaction domain with C-terminal coactivators (tubArm-ΔC) only partially restore Sens expression levels. The double-mutant form of Arm (tubArm-dm) completely lacks the ability to restore Sens expression.