Skip to main content
. 2011 Dec 15;25(24):2631–2643. doi: 10.1101/gad.181289.111

Figure 4.

Figure 4.

Developmental defects and reduction in β-catenin-mediated transcription in embryos expressing the D164A mutant form. (A) Wild-type (wt) and homozygous β-cateninD164A/D164A (D164A) embryos at E10.5 carrying one allele of the Wnt/β-catenin transcriptional reporter BAT-gal and stained for enzymatic activity with X-gal (blue). The mutant embryos show severe defects in size and in the development of brain, craniofacial structures, and pharyngeal arches. These defects are associated with reduced BAT-gal activity. Representative embryos are shown. (B) Wnt/β-catenin transcription is reduced in such mutant embryos as determined by quantitative real-time PCR in the head and the pharyngeal arches at E10.0. Expression of the BAT-gal reporter and of the endogenous Wnt/β-catenin target gene Axin2 in the head, pharyngeal arches, and the rest of the body was monitored. Transcript levels are normalized to those of the housekeeping genes SDHA and GAPDH. Levels for wild-type (wt) embryos are set to 1. Error bars show standard deviations. Representative results are shown.