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. Author manuscript; available in PMC: 2011 Dec 30.
Published in final edited form as: Arch Ophthalmol. 2011 Apr 11;129(8):1030–1041. doi: 10.1001/archophthalmol.2011.75

Figure 7. Surgical removal of the developing eye alters EOM formation.

Figure 7

A) In situ hybridization for crestin expression demonstrates that neural crest is adjacent to the neural tube at 16 hpf (left) and has migrated to peripheral locations by 30 hpf (right). B) H&E staining of a transverse section of 5 month old zebrafish that was enucleated at 16 hpf reveals one rectus muscle close to the origin, but a complete lack of other rectus and oblique muscles in the anophthalmic orbit. (scale bar 250μm)