Figure 1.

Caecal global microRNA expression is correlated in germ-free and conventional mice. Scatter plot depicting the relationship between global miRNA expression levels of 336 miRNAs in germ-free (GF) and conventional (Conv) caecal samples (n=3/group) as assessed by qRT-PCR and TLDA plates A and B. Data are presented as mean delta Ct values for each miRNA (mean delta Cts represent the average delta Ct for all three samples/group, with each individual Ct normalized by mean expression value normalization procedure). Of the 585 miRNA species analyzed, 336 remained after filtering and normalization, as explained in Materials and Methods and were used in subsequent analyses.