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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Pharmacogenomics J. 2010 Apr 13;11(4):274–286. doi: 10.1038/tpj.2010.28

Figure 7.

Figure 7

In vitro cell transfection assays. (a) Effect of intron 6 region on CYP3A4-promoter activity tested in a luciferase reporter gene assay. The intron 6 region was inserted upstream of the proximal CYP3A4-promoter region, and both C and T alleles were tested using bioluminescence output. (b) Effects of intron 6 SNP on CYP3A4 minigene RNA expression. Minigene constructs, consisting of exon 6, intron 6, and exon 7, harboring the C or T allele of intron 6 SNP, were co-transfected into HepG2 or HEK293 cells, and allelic DNA and RNA ratios measured at 24, 48, and 96 h post-transfection, using intron 6 SNP as the marker. The intron 6 SNP plasmid DNA ratio was normalized to 1 for each experiment. Compared with plasmid DNA ratio, **P<0.01, ***P<0.001 analysis of variance with Bonferroni post-test.