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. 2012 Jan 1;23(1):45–58. doi: 10.1091/mbc.E11-05-0434

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© 2012 Chin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Premature MEN but not FEAR activation in Noc-treated cells can cause Chs2 ER export. Wild-type (i), cdc55Δ (ii), or bub2Δ (iii) cells harboring CDC14-mCHERRY CHS2-GFP were released from α-factor–induced G1 into YPD + Noc. Cells were observed every 30 min from 120 min after G1 release when cells were in metaphase. Each cell counted was evaluated for Chs2-GFP and Cdc14-mCherry localization. Chs2-GFP released refers to absence of ER signals. Cdc14-mCherry dispersal was taken as loss of distinct nucleolar signals. Percentages of cells showing Cdc14 cytoplasmic dispersal and Chs2 neck localization are plotted.