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. 2012 Jan 1;23(1):59–70. doi: 10.1091/mbc.E11-06-0487

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© 2012 Chu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — UHRF1 levels are tightly regulated, and phosphorylation of UHRF1 at Ser-661 is essential for zebrafish embryogenesis. (A) Western blot with α-myc of 6, 12, and 24 hpf embryos injected with 400 pg of mRNA encoding full-length WT-UHRF1 and UHRF1^S661A. Tubulin is the loading control. (B) Both depletion and overexpression of UHRF1 are deleterious to embryonic survival. Only 57% of morphants and 61% of embryos injected with 6X-myc-UHRF1 survive to 50% epiboly. Survival improves to 81% when the morpholino is coinjected with the 6X-myc-UHRF1 mRNA (compare columns 2–4 and columns 3–4; for both, p < 0.0001 by Fisher's exact test). Coinjection of morpholino and mRNA encoding UHRF1^S661A mRNA results in no difference in mortality as compared with morpholino alone; p = 0.9146 by Fisher's exact test. mRNA = 400 pg. The total number of embryos and experiments are noted under each bar.