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. 2012 Jan 1;23(1):99–110. doi: 10.1091/mbc.E11-06-0497

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© 2012 Smith et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — ROCK1 is recruited to AJs. Immunofluorescence analysis of E-cadherin (green) and ROCK1 (red) in A431 cells during a calcium-switch assay. Cells were fixed and stained under the following conditions: (A) no calcium depletion (control), (B) depletion of calcium using LCM, (C) 10-min postcalcium restoration, (D) 30-min postcalcium restoration (arrows indicate low levels of ROCK1 at cell junctions), (E) 60-min postcalcium restoration, (F) 60-min postcalcium restoration with incubation with E-cadherin– and P-cadherin–blocking antibodies (HECD-1 and 6A9). Scale bars: 10 μm.