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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Biosens Bioelectron. 2011 Oct 25;31(1):310–315. doi: 10.1016/j.bios.2011.10.037

Figure 2.

Figure 2

(A) The optical responses of 1-nM R156H-mutant DNA on the LFSs with different ISDPR solutions. Solution 1: 5.0×10−8 M biotin-modified hairpin probe, 5.0×10−8 M digoxin-modified primer, 3-U polymerase Klenow fragment exo−, 50-µM dNTPs, 6% DMSO, 0.1% BSA, 1-mM DTT, and 5 mM MgCl2 in 50-mM Tris–HCl (pH 8.0) buffer; Solution 2: solution 1 without polymerase Klenow fragment exo−; Solution 3: solution 1 without dNTP; Solution 4: solution 1 without biotin-modified hairpin probe; and Solution 5: solution 1 without digoxin-modified primer. ISDPRs were performed 1 h at 42°C. Corresponding photo images of the LFSs are shown in the inset. (B) The effect of ISDPR time on the S/N ratio of assays. (C) Gel image of ISDPR products in the absence (lane a) and presence of 1-nM R156H-mutant DNA. Lane a: product generated in the absence of R156H-mutant DNA with an ISDPR time of 60 min. Lanes b–d: products generated in the presence of 1-nM R156H-mutant DNA at different ISDPR time intervals: (b) t = 0 min, (c) t = 15 min, (d) t = 30 min, and (e) t = 60 min. Lane e: DNA ladder markers (10, 20, 30, 40, 50, 60, 70, 80, and 90 bp from the bottom). ISDPRs were performed with solution 1 in (A) at 42°C.