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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Pacing Clin Electrophysiol. 2011 Sep 25;35(1):3–16. doi: 10.1111/j.1540-8159.2011.03222.x

Figure 2. Baseline Protein Expression of G816V HERG compared to WT HERG in HEK 293 cells.

Figure 2

A) Western blot shows whole cell lysate of HEK 293 cells transfected with either WT HERG or G816V HERG plasmid DNA alone or in a 50/50 mix at 48 and 72 hours post-transfection. Antibodies used were against the Myc-tag for both types of HERG and against Na+/K+ ATPase as a loading control. The molecular weight marker appears at left and HERG appears as a doublet of two bands at 135 kDa and 155 kDa (arrows) separated on 7.5% SDS-PAGE. B) Histogram showing summary data of total HERG protein expression levels normalized to Na+/K+ ATPase. Graph shows densitometry quantification of total HERG where protein expression of total G816V HERG compared to total WT HERG is reduced both at 48 and 72 hours, n=5 with ** p-value < 0.01 and ***p-value < 0.001. C) Histogram showing the distribution of 135 kD and 155 kD HERG bands for wild-type, 50–50 mix and G816V with totals normalized to 1.