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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Pacing Clin Electrophysiol. 2011 Sep 25;35(1):3–16. doi: 10.1111/j.1540-8159.2011.03222.x

Figure 5. Interaction of WT HERG and G816V HERG subunits.

Figure 5

A) Co-immunoprecipitation and subsequent Western blot from transiently transfected HEK 293 cells shows 3x FLAG-tagged WT HERG detected from a pull-down of myc-tagged G816V HERG in 3 different mixed combinations with 100% WT HERG and 100% G816V HERG as controls, n=3. Lower panel shows the reverse experiment where myc-tagged G816V HERG was detected in a pull-down of 3x FLAG-tagged WT HERG from the same combinations, n=3. B) Immuno-blots of cell surface biotinylation of 3x FLAG-tagged WT HERG and myc-tagged G816V HERG alone or in a 50/50 mix. Proteins were separated by 7.5% SDS-PAGE. Top panel shows a blot with anti-FLAG antibody with cadherin and calnexin as controls. Lower panel shows a blot with anti-Myc antibody with cadherin and calnexin as controls, n=3.