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Reaction schematic illustrating the strategy employed for tethering of MFZ2-12 to the AFM tips. The heterobifunctional cross-linker maleimide-PEG27-NHS was linked to the AFM tip. The NHS ester function supports the coupling of the linker to the amine groups on the tip. These amine groups were introduced with APTES from the gas phase. Subsequently, MFZ2-12-PDP (prepared from MFZ2-12-NH2 and SPDP) was allowed to react with the terminal maleimide group.
