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. 2011 Nov 14;287(1):148–158. doi: 10.1074/jbc.M111.298117

FIGURE 7.

FIGURE 7.

p130CAS and ERK5 are crucial for forming peripheral adhesion complexes. A, p130CAS protein is knocked down by siRNAs at day 3 and re-expressed at day 6. PC3 cells were transfected with p130CAS siRNAs or control siRNAs and harvested at 3 or 6 days. Total cell lysates were analyzed by immunoblotting with anti-p130CAS, Myc tag (PTK6), and β-catenin antibodies. B, Palm-PTK6-YF-induced peripheral adhesion complexes are disrupted upon p130CAS knockdown (si-CAS) and reassembled once p130CAS is re-expressed at day 6. Phase-contrast images and indirect immunofluorescence using anti-phosphotyrosine (PY) antibodies are shown. Size bar denotes 50 μm. C, ERK5 protein is knocked down by two ERK5 siRNAs. PC3 cells were transfected with ERK5 siRNAs (si-E5) or control siRNAs (si-Cont) and harvested at 3 days. Total cell lysates were analyzed by immunoblotting with anti-ERK5, phospho-ERK5, Myc tag, and β-catenin antibodies. D, peripheral adhesion complexes are disrupted upon ERK5 knockdown. Phase-contrast images and indirect immunofluorescence using anti-phosphotyrosine antibodies are shown. Size bars denote 50 μm. Vec, vector.