FIGURE 3.

PkPSD cDNA complements the Etn auxotrophy of the psd1psd2dpl1 strain. a, wild type, psd1Δpsd2Δdpl1Δ mutant, and psd1Δpsd2Δdpl1Δ mutant strains harboring pBEVY-PkPSD were inoculated into synthetic glucose medium supplemented with 2 mm Etn and grown overnight. Cells were harvested and reinoculated at an A₆₀₀ = 0.005 in synthetic glucose medium plus or minus 2 mm Etn as indicated. Cell growth at 30 °C was monitored by A₆₀₀. b and c, psd1Δpsd2Δdpl1Δ mutant strains harboring an empty vector, YEp352-ScPSD1, or pBEVY-PkPSD were cultured in SC-U (b) or SL-U (c) medium with or without 2 mm Etn as indicated. 5-Fold serial dilutions of cells were plated onto the medium and incubated at 30 °C for 2 days (b) and 3.5 days (c).