TABLE 1.
Parameters generated by simultaneous fitting of multiple nSLD profiles
Data were collected after 180-min equilibration. d, layer thickness in Å; Г, surface excess of each component at the interface compared with the aqueous bulk. Where the summed percentages for a layer exceed 100%, this is within the overall error for the layer (e.g. OmpF + ColN + DPPG lipid headgroup layer = 101.8 ± 6.7). These results, obtained by not constraining the fits to 100%, emphasize the quality of the models. Where totals are significantly less than 100%, the difference reflects the water content of the layer.
| Layer number | DPPG |
ColN + DPPG |
OmpF + DPPG |
OmpF + ColN +DPPG |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| d | Lipid | Г | d | Lipid | ColN | Г | d | Lipid | OmpF | Г | d | Lipid | OmpF | ColN | Г | |
| Å | % | mg/m2 | Å | % | % | mg/m2 | Å | % | % | mg/m2 | Å | % | % | % | mg/m2 | |
| 1 (protein only/air) | 18.0 ± 2.0 | 8.2 ± 2.3 | 0.34 ± 0.1 | 16.7 ± 0.06 | 8.2 ± 2.4 | 5.3 ± 3.1 | 0.6 ± 0.21 | |||||||||
| 2 (lipid acyl chain) | 17.2 ± 1.77 | 98 ± 5.2 | 1.73 ± 0.09 | 15.8 ± 1.0 | 97.0 ± 3.0 | 3.7 ± 1.6 | 1.64 ± 0.049 | 17.2 ± 0.13 | 69 ± 2.1 | 10.5 ± 1.7 | 1.58 ± 0.10 | 20.4 ± 0.5 | 69 ± 10 | 10.8 ± 4.6 | 11.6 ± 6.0 | 2.26 ± 0.41 |
| 3 (lipid headgroup) | 6.15 ± 0.5 | 74 ± 7.2 | 0.8 ± 0.078 | 8.0 ± 0.01 | 67.0 ± 3.5 | 21 ± 6.0 | 1.31 ± 0.069 | 5 ± 0.5 | 82 ± 0.2 | 8.2 ± 2.1 | 0.8 ± 0.004 | 5.0 ± 0.08 | 82.0 ± 5.0 | 8.2 ± 4.2 | 11.6 ± 1.7 | 1.1 ± 0.3 |
| 4 (protein only/water) | 60.5 ± 2.2 | 40 ± 1.8 | 5.4 ± 0.24 | 10.0 ± 1.0 | 1.0 ± 6.0 | 0.03 ± 0.13 | 50.1 ± 4.1 | 0.5 ± 0.5 | 11.6 ± 1.5 | 1.34 ± 0.17 | ||||||