TABLE 1.
Apparent dissociation constant (Kd) values for the interaction of TCP proteins and their respective mutants with C20 and C16
Binding curves were fitted to the Hill equation with a Hill number of 2, assuming that dimer formation is required for DNA binding. ΔKd indicates the S.D. of three independent experiments. Values indicate concentration of TCP monomers. The root mean square deviation (r.m.s.d.) errors and correlation coefficients (r2) of the fits to the experimental data are also indicated.
| Protein | C20 |
C16 |
||||||
|---|---|---|---|---|---|---|---|---|
| Kd | ΔKd | r.s.m.d. | r2 | Kd | ΔKd | r.s.m.d. | r2 | |
| nm | nm | % | nm | nm | % | |||
| TCP16 | 81.2 | 7.2 | 12.3 | 0.980 | 49.2 | 2.2 | 3.2 | 0.996 |
| D11G-TCP16 | 238.6 | 8.4 | 10.7 | 0.962 | 239.3 | 25.1 | 10.9 | 0.961 |
| TCP20 | 122.0 | 5.6 | 9.7 | 0.956 | 169.1 | 13.8 | 14.5 | 0.892 |
| G11D-TCP20 | 138.4 | 1.0 | 9.6 | 0.970 | 68.4 | 5.1 | 11.6 | 0.939 |
| TCP4 | 157.4 | 13.9 | 9.8 | 0.932 | 99.1 | 7.1 | 10.6 | 0.944 |
| D15G-TCP4 | 162.6 | 23.1 | 6.1 | 0.983 | 162.6 | 1.5 | 7.2 | 0.979 |