FIGURE 1.

Omentin promotes perfusion recovery and capillary vessel formation of ischemic limbs in mice in vivo. Ad-omentin or Ad-β-gal (control) was injected into the jugular vein of WT mice (2 × 10⁷ pfu in each group) 3 days prior to surgery. A, representative LDBF images in ischemic limb of Ad-omentin-treated or control mice (left panels). Quantitative analysis of the ischemic/non-ischemic LDBF ratio in Ad-omentin-treated and control WT mice before surgery, after surgery, and on postoperative days 3, 7, and 14 (right panel). Results are shown as the mean ± S.D. (n = 7). B, fluorescence staining of ischemic tissues with anti-CD31 monoclonal antibody (green; left panel). Quantitative analysis of capillary density in Ad-omentin-treated and control mice on postoperative day 14. Capillary density was expressed as the number of capillaries per high power field (×400, middle panel) and capillaries per muscle fiber (right panel). Results are shown as the mean ± S.D. (n = 5).