FIGURE 2.

N terminus of Rictor is dispensable for acetylation. A, Rictor truncations are diagrammed. B, Rictor co-immunoprecipitation of mTOR, mSin1.1, and LST8 requires both termini. HEK 293T cells were transfected with or without mSin1.1 and various Rictor proteins. Rictor was immunoprecipitated (IP) using α-FLAG (FL) antibodies or α-HA antibodies as indicated. Co-immunoprecipitants were analyzed by immunoblotting (IB). C, acetylation of overlapping Rictor truncations highlights central region containing residues 957–1188. Acetylation assays were performed with various Rictor proteins. Proteins were immunoprecipitated as described in B and analyzed by immunoblotting. Data shown are representative examples of two independent experiments performed per panel.