FIGURE 4.

Wogonin, apigenin, and chrysin enhance p53 expression in ATL cells via inhibition of Mdm2 expression. A, wogonin down-regulates p53 mRNA but up-regulates p53 protein expression in ATL cells. SP and MT-2 cells were treated with 50 μm wogonin for different time periods as indicated. The effect of wogonin on p53 expression was monitored by quantitative PCR (left) and Western blot analysis (right). Data are representative of two independent experiments. Means ± S.D. (error bars) are shown. B, wogonin suppresses Mdm2 mRNA and protein expression in ATL cells. SP and MT-2 cells were treated with 50 μm wogonin for different time periods as indicated. The effect of wogonin on Mdm2 expression was monitored by quantitative PCR (left) and Western blot analysis (right). Data are representative of two independent experiments. Means ± S.D. are shown. C, wogonin, apigenin, and chrysin inhibit Mdm2 but up-regulate p53 expression in ATL cells. MT-2 and SP cells were treated with different concentration of flavones for 16 h. Mdm2 and p53 expression levels were examined by Western blot analysis. Data are representative of two independent experiments. D, model of the mechanism by which flavones sensitize TRAIL-induced apoptosis.