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. 2011 Nov 15;287(1):672–681. doi: 10.1074/jbc.M111.317370

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Dsc5 and Cdc48 interact with Dsc2. Digitonin-solubilized extracts were prepared from cross-linker-treated, wild-type, and dsc2-13xMyc anp1-GFP cells. Dsc2-13xMyc and its interacting proteins were immunopurified (IP) with anti-Myc IgG-9E10 monoclonal antibody. Equal amounts of input (lanes 1 and 2), unbound (lanes 3 and 4), and 10-fold bound (lanes 5 and 6) fractions were analyzed by immunoblot with anti-Dsc1 IgG, anti-Myc IgG, anti-Dsc3 serum, anti-Dsc4 serum, anti-Dsc5 serum, anti-Cdc48 serum, and anti-GFP IgG.