FIGURE 5.

Cytotoxicity of stabilized protein oligomers is inhibited by DNA and heparin. RPMI 8226 cells cultured with 10 μg/ml HSA-derived proteins in the presence of 10 μg/ml DNA or 10 μg/ml heparin for 24 h. The viability of the culture cells were examined by (A) Trypan Blue, (B) CellTiter-Blue®, and (C) PI staining. D, dead cell populations in RPMI 8226 cells cultured with different amounts of prion peptide in the presence of control, 10 μg/ml DNA, or 10 μg/ml heparin was assessed by PI staining.