FIGURE 6.

EGCG inhibits conversion of stabilized protein oligomers to amyloid. A, HSA or EDC-crosslinked HSA were pre-incubated with increasing amounts of EGCG. Dot blot analysis was performed with A11 antibody. B, EDC-crosslinked HSA (10 μg/ml) was pre-incubated with increasing amounts of EGCG then added to the culture of RPMI 8226 cells. After 24 h, the percentage of dead cell population was measured by PI staining. C, EDC-crosslinked HSA was pre-incubated with increasing amounts of EGCG then mixed with heparin. Emission by Thioflavin T at 486 nm after adding to the resulting protein samples was measured. Fluorescence emission by the control buffer was used as a reference. Relative fluorescence emission of other samples was calculated and plotted. Error bars are means ± S.D.