FIGURE 3.

Combined knockdown of Arf4 and Arf5 decreases the release of the four dengue serotype RSPs. A, cell lysates (CL) and supernatants (SN) were prepared from HeLa-prME DENV1, DENV2, DENV3, and DENV4 cells transfected with either NT or siRNA targeting Arf4 + 5. Samples were analyzed by Western blot using the 4G2 monoclonal antibody that recognizes all four DENV serotypes (28). The results shown are representative of two independent experiments. B, mean density of each RSP band was measured using Photoshop, and the relative amount of RSPs was then calculated by normalizing it to that of NT siRNA. Results are shown as mean ± S.D. of triplicate measurements from one experiment representative of three others.