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Fusion is increased in the presence of the BiFC tags, but the relative fusion properties of wt and variant HNs are maintained. A–C, 293T cells were co-transfected with the indicated HN and F, and fusion was analyzed as described under “Experimental Procedures.” D–F, relative fusion between wt HN and H552Q HN is maintained irrespective of the tag. RLU, relative luminescence units; EGFP, enhanced green fluorescent protein.
