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. 2011 Nov 22;287(1):778–793. doi: 10.1074/jbc.M111.298059

FIGURE 8.

FIGURE 8.

Hyper-triggering HN mutant with mutation in site II at dimer interface (H552Q) shows enhanced homo-oligomerization. A, shown are mean fluorometric ratios of BiFC resulting from oligomerization of HN WT-HN WT and HN H552Q-HN H552Q. Data are the means of three-four readings from triplicate experiments with S.E. B, shown is a schematic of untagged competition BiFC experiments using untagged HN to displace tagged HN in HN-HN oligomerization. C, 293T cells were co-transfected with HN WT N-Venus, HN WT C-Venus, and pCAGGS (BiFC (WT HN+WT HN)), HN WT N-Venus, HN WT C-Venus, and HN H552Q pCAGGS (BiFC (WT [HN+HN]) + cold HN H552Q), or HN WT N-Venus, HN WT C-Venus, and HN WT pCAGGS (BiFC (WT [HN+HN] + cold HN WT) or HN H552Q N-Venus, HN H552Q C-Venus, and pCAGGS (BiFC (H552Q[HN+HN])), HN WT N-Venus, HN WT C-Venus, and HN H552Q pCAGGS (BiFC (H552Q[HN+HN]) + cold HN H552Q), or HN H552Q N-Venus, HN H552Q C-Venus and HN WT pCAGGS (BiFC (H552Q[HN+HN]) + cold HN WT). Zanamivir (10 mm) was added to prevent HN-receptor interaction. One hour before analysis, fresh 10 mm zanamivir and cycloheximide were added. **p < 0.01, ***,p < 0.001 (one-way analysis of variance). Data are the means of three-four readings from triplicate experiments with S.E.