Fig. 9.

The RT-PCR and Western blot analysis showing induction of cyclin D1 mRNA and protein after Il-7 stimulation and reduction in cyclin D1 mRNA and protein after blocking IL-7R or inhibiting AP-1 in nude mice (a, b). Western blot analysis showing up-regulation of c-Fos, c-Jun, and p-c-Jun proteins in nude mice treated with IL-7, and blocking IL-7R with IL-7R-specific antibody (sc-662) or inactivating AP-1 with AP-1 inhibitor (SP600125) down-regulating the expression of c-Fos, c-Jun, and p-c-Jun proteins (c). IL-7 could induce the formation of AP-1 (c-Fos/c-Jun) heterodimmer via IL-7R with CoIP method in vivo (d). The CHIP analysis showing that AP-1 (c-Fos/c-Jun) could bind with cyclin D1 promoter, and the activity of binding were enhanced by IL-7 in xenograft tumors (e)