Fig. 4a–b.

Typical results from the simultaneous detection of L. monocytogenes and generic Listeria spp. amplicons by agarose gel electrophoresis (a) and NALFIA (b) after duplex PCR. For both a and b, chromosomal DNA of L. monocytogenes (lane 1), L. innocua (lane 2), and Enterobacter cloacae (lane 3) were used for the duplex PCR. The negative control (lane 4) is a primer control (PCR without template DNA). Mr DNA size marker, TL1 test line specific to all species from the genus Listeria (line with anti-FITC antibody), TL2 test line specific to L. monocytogenes (line with anti-DIG antibody). Reproduced from [47]