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The effect of the KLN-205 cell culture density on the amount of FBPase. The culture confluence was determined using a MTT-based assay. The amount of FBPase was estimated using ELISA with the secondary antibodies conjugated to peroxidase and detected with TMB. The changes in FBPase amount in response to increasing density of the KLN-205 cell culture were expressed as a TMB/MTT absorbance ratio. The results of three experiments are presented as a mean and SD; p < 0.05
