Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Oct 9;18(11):1204–1210. doi: 10.1038/nsmb.2139

Figure 6.

Figure 6

DKK1 residues in DKK1–LRP5/6 interface are important for its inhibitory effects on Wnt/β-catenin signaling. Conditioned media was generated for each of the indicated DKK1 constructs and each was tested for its ability to inhibit Wnt3A mediatedβ-catenin signaling in RKO cells as measured by a β-catenin activated reporter (BAR). (a) mutations on interface A; (b) mutations on interface B. Assays were performed in triplicate and error bars represent standard deviation. Reporter data were normalized to expression of the corresponding DKK1 WT or mutant protein as determined by desitometric analysis of a western blot (Supplementary Fig. 6).