Figure 1 .

Vegetative incompatibility (vic) genotyping of C. parasitica strain EP146. Strains of C. parasitica that differ at one or more vic loci form barrage lines (a line of dead cells) when colonies of the two strains merge as observed for the pairing of strains EP155 and EP146 (barrage line indicated by red arrow at bottom of the culture plate). Cortesi and Milgroom (1998) established a collection of 64 C. parasitica tester strains that represent all possible genotypes arising from the six genetically determined vic loci. Strain EP155, the strain used for generation of the reference genome sequence (http://genome.jgi-psf.org/Crypa2/Crypa2.home.html), was previously reported (Cortesi and Milgroom 1998) to give a compatible reaction (fusion of hyphae with no barrage formation) with tester strain EU-5 (vic genotype 2211-22) as shown at the left of the culture plate. Resequenced strain EP146 was found to be compatible with tester strain EU-17, as shown at the right side of the culture plate, and thus to have the vic genotype 2112-11.