. 2012 Jan;190(1):79–90. doi: 10.1534/genetics.111.132779

Table 1 . Integration of regulators and conditions that affect hilA expression into SPI1 regulatory circuit.

Class	Regulator	Medium, growth conditions	Mode of regulation (positive or negative regulator)	Fold effect of null mutation or change in growth conditions	Regulation of hilA via HilD	Regulation of hilD–lac transcriptional fusion	Regulation of hilD’-‘lac translational fusion
I	HilE	HSLB	−	4× ↑	Yes	No	No
	FliZ	HSLB	+	4× ↓
	EnvZ	HSLB	+	3.5× ↓
	FadD	HSLB	+	3× ↓
II	SirA	HSLB	+	3× ↓	Yes	Yes/no	Yes
	Dam	HSLB	+	5× ↓
	YfgL	HSLB	+	5× ↓
	Ack Pta	HSLB, with MOPS pH 6.0	+	2× ↓
	TrkA	HSLB	+	2.5× ↓
III	PhoPQ (phoQ24)	HSLB	−	10× ↓	No	No	No
	Dimethyl sulfide	HSLB, 1.5% DMS	−	2.5× ↓
	Fnr	HSLB	−	2× ↑			(↓)
IV	H-NS^a	NSLB	−	↑	No	Yes	Yes
	Hha	HSLB	−	3.5× ↑
	Fis	HSLB	+	15× ↓
	HU	HSLB	+	4.7× ↓
	RfaH	HSLB	+	4× ↓
	Butyrate	HSLB, 10 mM butyrate	−	3× ↓
	ppGpp (relA spoT)	HSLB	+	22× ↓
V	Fur	HSLB	+	5× ↓	Yes	No	Yes

HSLB, high salt LB broth—standard SPI1 inducing conditions as described in Materials and Methods; NSLB, no salt LB broth.