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. 2012 Jan 2;2:83. doi: 10.3389/fphar.2011.00083

Figure 2.

Figure 2

PDBu-stimulated MLC phosphorylation levels in bladder smooth muscle. Intact strips of bladder smooth muscle were contracted for 5 min with PDBu (3 μM) in the presence or absence of Bis (3 μM) or H-1152 (1 μM). Strips were rapidly frozen at 0, 1.5, 3, and 5 min of contraction and then processed for quantification of MLC phosphorylation levels. (A) PDBu stimulation significantly increased MLC phosphorylation levels at 1.5 and 3 min (•). MLC phosphorylation levels in strips incubated with either Bis (o) or H-1152 (▼) prior to PDBu stimulation were not significantly greater than basal values. (B) Percent increase in MLC phosphorylation levels from basal values in response to PDBu alone (•) or after 20 min incubation in Bis (o) or H-1152 (▼). Values shown are the means ± SE of 8–10 determinations. #P < 0.05, as compared with values at 0 min of the same treatment. *P < 0.05, as compared to PDBu alone at the same time point.