Figure 1.

Predicted methylation level of C. gigas genes categorized by biological processes compared to measured level of DNA methylation. Mean CpG O/E for 10,699 C. gigas genes categorized according to Biological Process Gene Ontology (GO) Slim terms are plotted on the x-axis (modified from Gavery and Roberts, 2010). DNA methylation was empirically measured by performing MBD-seq on the SOLiD 4 platform (Applied Biosystems). Genes identified in the MBD-library were associated with respective GO terms and enrichment analysis was performed based on the entire transcriptome (Fleury et al., 2009) using DAVID (Huang et al., 2009a,b). Results indicate the most underrepresented genes in the library are involved in cell adhesion and genes involved in DNA and protein metabolism were most prevalent in the MBD-library.