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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Virology. 2011 Oct 24;421(2):235–244. doi: 10.1016/j.virol.2011.09.032

Figure 2.

Figure 2

Western blot analyses of Env (i.e., gp160 and gp120) and Gag (i.e., p24) of Q461e2-associated pseudoviral supernatants. (A) Western blot of Q461e2-associated pseudoviral supernatants probed with a combination of anti-Env polyclonal rabbit primary Ab, which recognizes uncleaved Env (i.e., gp160) and cleaved Env (i.e., gp120), and anti-p24 mouse Ab for the detection of Gag (i.e., p24). The Env tested is shown above and the % Env cleavage is shown below. Percent cleavage was calculated as the amount of gp120/(gp120 + gp160). (B) Comparison of Env levels of mutants relative to Q461e2. For each variant (designated below bar graph) the total, cleaved, and uncleaved Env levels were calculated relative to Q461e2, which thus has a level of 1. (C) Comparison of Env/p24 levels of mutants relative to Q461e2. For each variant (designated below the bar graph) the total, cleaved and uncleaved Env levels divided by p24 levels are shown relative to Q461e2.