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. Author manuscript; available in PMC: 2012 May 18.
Published in final edited form as: Mol Cell. 2011 Sep 29;44(4):667–678. doi: 10.1016/j.molcel.2011.08.027

Figure 2. ChIRP enriches for TERC RNA and detects TERC-associated telomere DNA and TCAB1 protein.

Figure 2

(A) TERC-asDNA probes retrieve ~88% of cellular TERC RNA and undetectable GAPDH. LacZ-asDNA probes retrieve neither RNAs. Mean ± s.d. are shown. (B) Effect of different crosslinking agents on ChIRP-southern. After 1% glutaraldehyde crosslinking, TERC retrieval co-purifies telomeric repeats, but not Alu repeats. (C) TERC ChIRP retrieves TCAB1, a known telomerase holocomplex chaperone proteins. As a negative control tubulin was not detected.