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. Author manuscript; available in PMC: 2012 May 18.
Published in final edited form as: Mol Cell. 2011 Sep 29;44(4):667–678. doi: 10.1016/j.molcel.2011.08.027

Figure 5. HOTAIR ChIRP-seq suggests mechanisms of HOTAIR-recruitment of PRC2.

Figure 5

(A) HOTAIR binding sites are enriched in genic regions, notably enhancers and introns. (B) Metagene analysis of genomic regions aligned by 832 HOTAIR ChIRP peaks show focal HOTAIR peaks in association with broad domains PRC2 occupancy (evidenced by subunits EZH2 and Suz12) and H3K27Me3. (C) HOTAIR nucleates broad domains of PRC2 occupancy. A HOTAIR binding site between HOXD3 and HOXD4 lies in the center of a broad domain of Suz12 and H3K27Me3 occupancy that are both lost upon HOTAIR knock down (Tsai et. al., 2010, Rinn et. al., 2007). (D) GA-rich homopurine motif enriched in HOTAIR binding sites.