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Effects of 17β-estradiol on IL-1β–induced MAPK and IκB-α phosphorylation in corneal fibroblasts. Cells were incubated in the absence or presence of 17β-estradiol (100 μM) for 12 h and then in the additional absence or presence of IL-1β (0.1 ng/ml) for 30 min. Cell lysates were then prepared and subjected to immunoblot analysis with antibodies to total or phosphorylated (P-) forms of ERK, JNK, p38 MAPK, or IκB-α. Data are representative of three independent experiments.
