Table 5. Subcellular localization of the proteins involved in the cell cycle in endothelial cells and in both controls (in vitro endothelial cells and epithelial cells of whole flat-mounted corneas).

	Endothelial cells			Epithelial cells
Target protein	Fresh	Stored	In vitro cultures	Fresh	Stored
Ki67	A	A	N	N	N
PCNA	N	N	N, N+C	N, C, N+C	N
MCM2	C	C	N, N+C	N	N
cyclin D1	C	C	N, N+C	C, N+C	N
cyclin E	N	N	C	N, C, N+C	N, C, N+C
cyclin A	C	C	N, N+C	C	N, C, N+C
p16	N	N	N, C, N+C	N, C, N/C	C
p21	A	A	N	N, C	N
p27	N	N	N, C, N+C	N, C, N+C	N, C, N+C

A: absence of detection; N: nuclear; C: cytoplasmic, N+C: both nuclear and cytoplasmic.