A) Schematic of experimental strategy to analyze array structure by immunofluorescence.
B) Simplified hierarchy of C. elegans kinetochore assembly. The KMN network is comprised of KNL-1, the MIS-12 complex, and the NDC-80 complex.
C) Chromatin-associated inner kinetochore components CeCENP-A and KNL-2 (top row), the microtubule-binding outer kinetochore protein NDC-80 and the spindle checkpoint kinase BUB-1 (bottom row) localize to opposing faces of LacO-containing extra-chromosomal arrays during prometaphase. Arrowheads point to the array; the boxed region is magnified below. Scale bar 1 µm (0.5 µm for magnified regions).
D) Immunofluorescence of the LacO-containing extra-chromosomal array (LacI) and DAPI staining during late prometaphase, early anaphase and telophase in embryos. Higher magnification view of the array (arrowhead) with KNL-2 staining is shown on the bottom. Scale bar 2 µm (0.5 µm for magnified regions).