Figure 4.

Localization of SEP-AMPAR exocytic events relative to the postsynaptic density. Wide-field image of SEP-AMPAR fluorescence (white) imaged in a region of a dendrite of a living pyramidal neuron in dissociated culture (top panels), and the same image overlaid with the localization of endogenous PSD-95 (displayed in red) determined by post hoc immunolabeling of neurons after chemical fixation (bottom panels). Selected 100 ms frames are displayed from a sequential image series, showing the initial image of the dendritic region (left), a transient exocytic event (middle), and a persistent exocytic event (right). The scale bar is in the bottom left, and the time stamp (in seconds) is in the bottom right. The entire kinetic series is included as supplemental movie 5 (available at www.jneurosci.org as supplemental material).