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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Oct 7;1819(1):1–15. doi: 10.1016/j.bbagrm.2011.09.006

Table 3.

Oligomers containing candidate TNIP1 promoter PPREs.

Predicted PPRE Top & Bottom
A ctagGAATGATGAAGAAAGGCCAGTTCATCCAAAg
ctagcTTTGGATGAACTGGCCTTTCTTCATCATTC
B ctaggCCTGGCTGACCTGTTCCCACTTCCAGGAGGg
ctagcCCTCCTGGAAGTGGGAACAGGTCAGCCAGGc
C ctagTCACTTTGCCCTTTCCTCTCCTCCAg
ctagcTGGAGGAGAGGAAAGGGCAAAGTGA

Oligomers are all in 5′ → 3′ orientation. Lower case nucleotides at oligomer ends indicate Nhe I compatible overhang, at 5′ end, and overhang and regeneration of Nhe I site, at 3′ end to allow for step-wise multimerization with subsequent PPRE oligomer. Nucleotides in capitals are from TNIP1 promoter sequence with DR1 core of predicted PPRE in bold type. The sequence for element C was also used in EMSA.