Survival of vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) transduced schistosomules in the presence of the aminoglycoside antibiotic G418/geneticin. (A) Percentage of live, VSVG MLV-transduced schistosomules and control non-virion exposed schistosomules cultured for 10 days in increasing concentrations of G418. Concentrations of G418 are indicated with discrete colors. ANOVA and Student’s t-test were performed among retrovirus transduced and control schistosomules cultured in the indicated concentration of G418. * P ≤ 0.05, ** P ≤ 0.01; control, control schistosomules; MLV, VSVG-MLV transduced schistosomules. (B) representative images of MLV transduced schistosomules (MLV 500) and control schistosomules (control 500) cultured for 8 days in the presence of 500 μg/ml G418. Scale bar, 200 μm. (C) neomycin resistance gene (neoR) copy number per ng of genomic DNA from VSVG-MLV transduced schistosomules cultured for 6 days in increasing concentrations of G418.