Figure 6.
Effect of benfotiamine on ERK1/2 phosphorylation and avtivation of p65 and Egr-1. Growth-arrested murine macrophages were treated with LPS without and with benfotiamine (100 μM) for indicated times at 37°C. Cell lysates were prepared and equal amounts of protein were subjected to western blot analysis using antibodies against A) ERK1/2, B) p65 and C) Egr-1. A representative blot from each group is shown. Data represent mean ± S.E. (n=3). # p<0.0001 Vs control; **p <0.0001 Vs LPS treated cells.