SUR1 and SUR2 protein expression in SUM159. (A) Whole cell lysates from the SUM159 cell line were analyzed via immunoblot analysis for SUR1 and SUR2. SUR1 protein levels were readily detected while SUR2 expression was low by comparison to MDA-MB-231, MDA-MB-435 and C8161.9 probed under the same conditions. (B) Quantified data from GJIC assays demonstrating changes in GJIC with increasing concentrations (10, 25, 50µM) of gliclazide, chlorpropamide, glimepiride and glibenclamide. Significant increases in GJIC were observed with glimepiride and glibenclamide treatments with no increases during treatment with gliclazide and chlorpropamide, similar to results obtained from MDA-MB-231, MDA-MB-435 and C8161.9.