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. 2011 Oct 25;415(1):61–74. doi: 10.1016/j.jmb.2011.10.032

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Copyright © 2011 Elsevier Ltd. All rights reserved.

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Fig. 5 — DDX1 ATPase activity is RNA dependent. (a) Spectroscopic ATPase activity of DDX1 in the presence of 15 μM stem IIB RNA measured as a function of ATP concentration. Michaelis kinetic hydrolysis constants (k_cat = 15.9 ± 0.7 min^− 1; K_m = 1.0 ± 0.1 mM ATP) were calculated from a fit of the data to the Briggs–Haldane equation. Increasing concentrations of either (b) 351-nt RRE or (c) stem IIB RNA were titrated into DDX1 in the presence of 2.5 mM ATP. Hydrolysis curves plot the rate of ATP hydrolysis as a function of RNA concentration.