Figure 3.

Nicotine-stimulated locomotor activity in six lines of α6 L9′S mice. A, B. Six lines of α6 L9′S mice were tested for nicotine-stimulated locomotor activity. Mice were habituated for 8 mins followed by nicotine administration (0.15 mg/kg; i.p.) at a dose designed to specifically activate hypersensitive α6* receptors. After injection, mice were monitored for an additional 30 mins. Average activity traces for groups of mice from each line are plotted with traces from control, non-transgenic mice. Results fell into two categories and were plotted on separate graphs for clarity: locomotor activity elevated compared to control (A), and locomotor activity slightly elevated or not elevated comparable to control (B). C. Plot of ambulations for nicotine-stimulated locomotor activity for each line compared to control. Mean ambulation values for control and six α6 L9′S lines are plotted for the peak response period (mins 9-20). The number of mice for nicotine-induced locomotor activity was as follows: control, n = 9; line 2, n = 7; line 5, n = 7; line 9, n = 8; line 11, n = 8; line 15, n = 8; line 29, n = 5. Data are mean ± SEM of untransformed data whereas p values are reported for Dunnett’s tests on square-root transformed data. * p < 0.05, *** p < 0.001