Figure 4.

Wheel running locomotor activity in six lines of α6 L9′S mice. A, B. Six lines of α6 L9′S mice were tested for acquisition of wheel running behavior over 9 d. Mice were habituated to cages fit with running wheels for 4 h, followed by continuous recording of running behavior for 9 d beginning at the onset of the dark phase each day. Total revolutions per day over 9 d for each α6 L9′S line are plotted compared to the same control group for comparison. The data for each group were fit to a single exponential function. Results fell into two categories and were plotted on separate graphs for clarity: wheel running activity depressed compared to control (A), and wheel running activity comparable to control (B). C, D. Daily wheel running time for six lines of α6 L9′S mice. Average time spent on the wheel per day is plotted for each α6 L9′S mouse line compared to control. Similar to (A) and (B), results were plotted on separate graphs for clarity: lines 2, 5, and 11 compared to control (C), and lines 9, 15, and 29 compared to control (D). The data for each group were fit to a linear regression function. E, F. Daily running speed for six lines of α6 L9′S mice. Average running velocity per day is plotted for each α6 L9′S mouse line compared to control. The data for each group were fit to a single exponential function. Results fell into two categories and were plotted on separate graphs for clarity: running velocity depressed compared to control (E), and running velocity comparable to control (F). The number of mice for wheel running was as follows: control, n = 18; line 2, n = 18; line 5, n = 10; line 9, n = 10; line 11, n = 20; line 15, n = 10; line 29, n = 10. Data are mean ± SEM.